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MABN40

Sigma-Aldrich

Anti-LRRK2 Antibody, clone N138/6

clone N138/6, from mouse

Synonym(e):

leucine-rich repeat kinase 2, DARDARIN, augmented in rheumatoid arthritis 17, Parkinson disease (autosomal dominant) 8, Dardarin

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

N138/6, monoclonal

Speziesreaktivität

human

Speziesreaktivität (Voraussage durch Homologie)

mouse (based on 100% sequence homology)

Methode(n)

immunohistochemistry: suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

NP_940980

UniProt-Hinterlegungsnummer

Q5S007

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... LRRK2(120892)

Allgemeine Beschreibung

Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.

Spezifität

This antibody recognizes LRRK2.

Immunogen

Recombinant protein corresponding to human LRRK2.

Anwendung

Anti-LRRK2 Antibody, clone N138/6 detects level of LRRK2 & has been published & validated for use in IH & WB.
Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (UC Davis/NIH NeuroMab Facility, www.neuromab.org, neuromab@ucdavis.edu)

Qualität

Evaluated by Immunohistochemistry in human placenta and Parkinson′s diseased vascular brain tissue.

Immunohistochemistry Analysis: 1:300 dilution of this antibody detected LRRK2 on 10 µg of human placenta and Parkinson′s diseased vascular brain tissue.

Zielbeschreibung

240 kDa observed. 286 kDa calculated. (Glasson, B., et al. (2006). Ann. Neurol. 59(2):315-322.)

Physikalische Form

Format: Purified

Hinweis zur Analyse

Control
Human placenta and Parkinson′s diseased vascular brain tissue

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Iakov N Rudenko et al.
The Biochemical journal, 474(9), 1547-1558 (2017-03-23)
Autosomal dominant mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson's disease (PD). Most pathogenic LRRK2 mutations result in amino acid substitutions in the central ROC (Ras of complex proteins)-C-terminus of ROC-kinase triple domain and affect enzymatic functions
Ruth Chia et al.
Nature communications, 5, 5827-5827 (2014-12-17)
LRRK2, a gene relevant to Parkinson's disease, encodes a scaffolding protein with both GTPase and kinase activities. LRRK2 protein is itself phosphorylated and therefore is subject to regulation by cell signalling; however, the kinase(s) responsible for this event have not

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