MABF2055

Anti-IDO-2 Antibody, clone 4-3

clone 4-3, from mouse

• Synonym(e): Indoleamine 2, 3-dioxygenase 2, Indoleamine 2, 3-dioxygenase-like protein 1, Indoleamine-pyrrole 2, 3-dioxygenase-like protein 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 4-3, monoclonal
• Speziesreaktivität: mouse
• Verpackung: antibody small pack of 25 μg
• Methode(n): western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_666061
• UniProt-Hinterlegungsnummer: Q8R0V5
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Ido2(209176)

Beschreibung

Allgemeine Beschreibung

Indoleamine 2,3-dioxygenase 2 (UniProt: Q8R0V5; also known as IDO-2, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1) is encoded by the Ido2 (also known as Indol1) gene (Gene ID: 209176) in murine species. IDO-2 is expressed mainly in antigen-presenting immune cells, liver, kidney, brain, and placenta. It is highly expressed in kidney, followed by epididymis and liver and is also detected in the tails of the spermatozoa. It catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. IDO-1 and IDO-2 are 2 distinct enzymes which catalyze the same reaction. However, the Km for IDO-2 for tryptophan is much higher than that of IDO-1. IDO-2 may play a role as a negative regulator of IDO-1 by competing for heme-binding with IDO-1. IDO-2 activity is inhibited by 1MT (1-methyl-tryptophan) and MTH-trp (methylthiohydantoin-DL-tryptophan). IDO-2 is also involved in immune regulation. IDO-2 knockout mice do not show any apparent defects in their embryonic development or hematopoietic differentiation and have wild-type profiles for kynurenine in blood serum and for immune cells in spleen, lymph nodes, peritoneum, thymus and bone marrow. However, the knockout mice exhibit defects in IDO-mediated T-cell regulation and inflammatory responses. (Ref.: Mbongue JC, et al. (2015). Vaccines (Basel). 3(3):703-29).

Spezifität

Clone 4-3 detects IDO-2 in mouse. It targets an epitope within 21 amino acids in the C-terminal region.

Immunogen

KLH-conjugated linear peptide/recombinant protein corresponding to 21 amino acids from the C-terminal region of murine Indoleamine 2,3-dioxygenase 2 (IDO-2).

Anwendung

Research Category
Entzündung & Immunologie

Anti-IDO-2, clone 4-3, Cat. No. MABF2055, is a mouse monoclonal antibody that detects IDO2 and has been tested for use in Western Blotting.

Western Blotting Analysis: 0.5 µg/mL from a represesntative lot detected IDO-2 in 10 µg of mouse kidney tissue lysate.

Qualität

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected IDO-2 in 10 µg of mouse liver tissue lysate.

Zielbeschreibung

~55 kDa observed; 45.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1