MABC1207

Anti-CIRP (CIRBP) Antibody, clone 6C7.1

clone 6C7.1, from mouse

• Synonym(e): Cold-inducible RNA-binding protein, A18 hnRNP, Glycine-rich RNA-binding protein CIRP
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 6C7.1, monoclonal
• Speziesreaktivität: rat, human
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_001271
• UniProt-Hinterlegungsnummer: Q14011
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CIRBP(1153)

Beschreibung

Allgemeine Beschreibung

Cold-inducible RNA-binding protein (UniProt Q14011; also known as A18 hnRNP, Glycine-rich RNA-binding protein CIRP) is encoded by the CIRBP (also known as A18HNRNP, CIRP) gene (Gene ID 1153) in human. CIRP is an 18-kDa RNA-binding protein with an RNA recognition motif (RRM; a.a. 6-84) and a glycine-rich domain (a.a. 92-158). CIRP migrates from nucleus to cytoplasm, where it mediates posttranscriptional regulation of target mRNAs. Originally characterized and named as a cold-induced protein, CIRP exerts antiapoptotic and cell-protective effects in response to cold stress, as well as other stimuli such as UV irradiation and hypoxia. CIRP is expressed in a broad range of tissues (e.g. testis, brain, lung, kidney, liver, stomach, bone marrow, and heart) and upregulated CIRP levels have been reported in several types of cancers. CIRP knockdown is reported to increase p53 level and enhance cancer cells chemo-sensitivity to DNA damage inducing drugs. Likewise, CIRP overexpression results in p53 downregulation.

Spezifität

Clone 6C7.1 is expected to recognize CIRP spliced isoforms 1 and 3 (UniProt Q14011). Cross-reactivity toward spliced isoform 2 is possible, but has not been confirmed.

Immunogen

GST-tagged recombinant human CIRP (CIRBP) internal fragment.

Anwendung

Research Category
Apoptose & Krebs

Detect CIRBP using this mouse monoclonal Anti-CIRP (CIRBP) Antibody, clone 6C7.1, Cat. No. MABC1207, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CIRP (CIRBP) in rat testis and human prostate cancer, pancreas, tonsil tissue sections.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CIRP (CIRBP) in 10 µg of Raji human B-cell lymphoma cell lysate.

Qualität

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CIRP (CIRBP) in 10 µg of SH-SY5Y human neuroblastoma cell lysate.

Zielbeschreibung

~18 kDa observed. 18.65/28.36/26.13 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable