MABC1099

Anti-uPAR (CD87) Antibody, clone 5D6

clone 5D6, from mouse

• Synonym(e): Urokinase plasminogen activator surface receptor, CD87
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 5D6, monoclonal
• Speziesreaktivität: hamster
• Verpackung: antibody small pack of 25 μg
• Methode(n): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: XP_007647182.1
• UniProt-Hinterlegungsnummer: Q9R119
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: hamster ... Plaur(100689422)

Beschreibung

Allgemeine Beschreibung

Urokinase plasminogen activator surface receptor (UniProt: Q9R119; also known as uPAR) is encoded by the Upar gene (Gene ID: 100689422) in Chinese hamster. uPAR is a GPI-anchored cell membrane receptor for urokinase plasminogen activator (uPA), an enzyme responsible for the activation of plasmin and degradation of extracellular matrix components. Higher expression of uPAR has been reported in many human cancers, including non-small cell lung cancer and colorectal cancer. Its higher levels correlate with poor prognosis and early invasion and metastasis. uPAR is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form, which contains three homologus domains (DI, DII, and DIII). uPAR is also reported to serve as an adhesion receptor for vitronectin and a direct interaction between uPAR and vitronectin is shown to be important for inducing changes in cell morphology, cell migration, and signaling. Blocking of uPAR activity can impair cell adhesion and migration in RAS mutated cancer cells. (Ref.: Di Mauro, C et al. (2017). Scientific Reports 7, Article number: 9388).

Spezifität

Clone 5D6 detects urokinase plasminogen activator surface receptor. It targets an epitope with in the extracellular domain.

Immunogen

Epitope: extracellular domain

Raft fraction of the plasma membrane from Chinese hamster ovary (CHO) cells.

Anwendung

Research Category
Apoptose & Krebs

Anti-uPAR (CD87), clone 5D6, Cat. No. MABC1099, is a mouse monoclonal antibody that detects Urokinase plasminogen activator surface receptor and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

Western Blotting Analysis: A representative lot detected uPAR (CD87) in Western Blotting applications (Maeda, Y., et. al. (2007). Mol Biol Cell. 18(4):1497-506; Lee, G.H., et. al. (2016). J Cell Biol. 215(5):705-718; Hirata, T., et. al. (2018). Nat Commun. 9(1):405).

Flow Cytometry Analysis: A representative lot detected uPAR (CD87) in Flow Cytometry applications (Ashida, H., et. al. (2006). J Biol Chem. 281(2):896-904; Lee, G.H., et. al. (2016). J Cell Biol. 215(5):705-718; Hirata, T., et. al. (2018). Nat Commun. 9(1):405; Hirata, T., et. al. (2013). J Biochem. 154(3):257-64).

Immunocytochemistry Analysis: A representative lot detected uPAR (CD87) in Immunocytochemistry applications (Kanzawa, N., et. al. (2012). J Lipid Res. 53(4):653-63).

Qualität

Evaluated by Flow Cytometry in Chinese hamster ovary (CHO) cells.

Flow Cytometry Analysis: 1 µg of this antibody detected uPAR (CD87) in one million Chinese hamster ovary (CHO) cells.

Zielbeschreibung

35.10 Da calculated.

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.