IM42

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

liquid, clone 114-6G6, Calbiochem^®

• Synonym(e): Anti-MMP-14
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 114-6G6, monoclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MMP14(4323)

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.

Recognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) is validated for use in Frozen Sections, Paraffin Sections, Immunoblotting, Immunocytochemistry, IP for the detection of MT1-MMP (Ab-3).

Immunogen

a synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP

Anwendung

Frozen Sections (not recommended)
Paraffin Sections (10 g/ml, Pronase<TMSYMBOL></TMSYMBOL> or heat pre-treatment required)
Immunoblotting (10 g/ml)
Immunocytochemistry (see application reference)
Immunoprecipitation (not recommended)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Negative Control
trpE (Ab-1) or untreated MD-MB-231 cells

Positive Control
HT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue

Sonstige Hinweise

Does not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.

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Goldberg, G.I., et al. 1989. PNAS86, 8207.

Rechtliche Hinweise

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1