HTS076RTA

Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells

Human

• UNSPSC-Code: 41106514
• eCl@ss: 32011203
• NACRES: NA.84

Eigenschaften

• product name: Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells, Human PAF GPCR frozen cells for Calcium Flux FLIPR Assays.
• Biologische Quelle: human
• Qualitätsniveau: 100
• Hersteller/Markenname: Ready-to-Assay
• Methode(n): calcium flux assay: suitable
• NCBI-Hinterlegungsnummer: NM_000952
• Nachweisverfahren: fluorometric
• Versandbedingung: dry ice

Beschreibung

Allgemeine Beschreibung

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Platelet-activating factor (PAF) is a bioactive phospholipid that was originally characterized by its ability to induce platelet aggregation, but was subsequently shown to mediate angiogenesis, inflammation and neural development. The PAF receptor is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and cytokine and leukotriene release (Stafforini et al., 2003). Millipore’s cloned human PAF-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAF expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAF and its ligands.

Beschreibung der Zelllinie

Host cells: Chem-1

Biochem./physiol. Wirkung

Protein Target: PAF

Target Sub-Family: Platelet Activating Factor

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable