DR1061

Anti-ENO1 Mouse mAb (8G8)

liquid, clone 8G8, Calbiochem^®

• Synonym(e): Anti-α Enolase 1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 8G8, monoclonal
• Form: liquid
• Enthält nicht: preservative
• Speziesreaktivität: human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ENO1(2023)

Beschreibung

Allgemeine Beschreibung

Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).

Purified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.

Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.

Immunogen

Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein

Anwendung

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (1 g/ml)

Warnhinweis

Toxicity: Regulatory Review (Z)

Physikalische Form

In PBS, pH 7.30.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
MCF-7 cells, Human lymphoma tissue, HeLa cells

Sonstige Hinweise

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Ho, J.A., et al. 2010. Anal. Chem.82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable