AP202A

Goat Anti-Rat light chain Antibody, Alkaline Phosphatase conjugate

Chemicon^®, from goat

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: goat
• Konjugat: alkaline phosphatase conjugate
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Speziesreaktivität: rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; western blot: suitable
• Isotyp: IgG
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Spezifität

Minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.

Immunogen

Epitope: Kappa light chain

Prepared from purified rat IgG light chain.

Anwendung

Research Category
Sekundär- & Kontrollantikörper

Research Sub Category
Fragmentspezifische Sekundärantikörper

Detect light chain using this Goat anti-Rat light chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.

Western Blotting: 1:5,000 -1:50,000

ELISA: 1:5,000 -1:50,000

Optimal working dilutions must be determined by the end user.

Zielbeschreibung

25 kDa

Physikalische Form

ImmunoAffinity Purified

Purified by immunoaffinity chromatography. Liquid in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.

Lagerung und Haltbarkeit

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3