ABS574-AF488

Anti-Surfactant Protein D Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• Synonym(e): Pulmonary surfactant-associated protein D, Collectin-7, Lung surfactant protein D, PSP-D, SP-D
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Konjugat: ALEXA FLUOR^™ 488
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human
• Methode(n): immunocytochemistry: suitable; immunofluorescence: suitable
• NCBI-Hinterlegungsnummer: NP_003010
• UniProt-Hinterlegungsnummer: P35247
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SFTPD(6441)

Beschreibung

Allgemeine Beschreibung

Pulmonary surfactant-associated protein D (UniProt P35247; also known as Collectin-7, Lung surfactant protein D, PSP-D, SP-D) is encoded by the SFTPD (also known as COLEC7, PSPD, SFTP4) gene (Gene ID 6441) in human. Surfactant, produced by alveolar type II cells and airway Clara cells, resides at the air-liquid interface of the lungs and is most commonly known for its role in reducing surface tension. Surfactant is a lipoprotein complex composed of approximately 10% proteins, including surfactant protein A (SP-A), SP-B, SP-C, and SP-D. SP-A and SP-D belong to the collectin family of proteins and can modulate innate immunity. SP-D is known to enhance pulmonary clearance of pathogens, such as Pseudomonous aerginosa, Klebsiella pneumonia, respiratory syncytial virus (RSV) and Influenza virus. SP-D is also shown to modify allergic responsee in the lungs by binding common allergens, including house dust mite and pollen particles. SP-A-deficient and SP-D-deficient mice are susceptible to bleomycin-induced lung injury and display increased cellular inflammation, more severe lung fibrosis, and reduced survival. In addition to lung, SP-D is also reported to be detected in extrapulmonary tissues, such as kidney and pancreas. Serum levels of either SP-A or SP-D in human are correlated with mortality in patients with pulmonary fibrosis. Human SP-D is produced with a signal peptide sequence (a.a. 1-20), which is removed posttranslationally to yield the mature protein (a.a. 21-375) that contains a collagen-like domain (a.a. 46-222), a coiled coil region (a.a. 223-252), and a C-type lectin domain (a.a. 260-375).

Spezifität

Target sequence shares 85.7% amino acid identity with the corresponding murine and rat region.

This Alexa Fluor^™ 488-conjugated polyclonal antibody targets a sequence within the C-type lectin domain of human surfactant protein D.

Immunogen

Epitope: domain of:

KLH-conjugated linear peptide corresponding to a sequence within the C-type lectin domain of human surfactant protein D.

Anwendung

Research Category
Zelluläre Signaltransduktion

Detect Surfactant Protein D using this rabbit polyclonal Anti-Surfactant Protein D Antibody, Alexa Fluor^™ 488 Conjugate, Cat. No. ABS574-AF488, validated for use in Immunocytochemistry and Immunofluorescence.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected surfactant protein D in human lung tissue sections.

The unconjugated antibody (Cat. No. ABS574) is also available for immunocytochemistry and Western blotting applications.

Qualität

Evaluated by Immunocytochemistry in A549 cells.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected surfactant protein D in A549 cells.

Zielbeschreibung

~48 kDa observed. 35.50/37.73 kDa (mature/pro-form) calculated.

Physikalische Form

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Rechtliche Hinweise

ALEXA FLUOR is a trademark of Life Technologies

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable