ABE432

Anti-BRDT Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Bromodomain testis-specific protein, Bromodomain-containing female sterile homeotic-like protein, RING3-like protein
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse
• Methode(n): immunohistochemistry: suitable
• NCBI-Hinterlegungsnummer: NP_473395
• UniProt-Hinterlegungsnummer: Q91Y44
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Brdt(114642)

Beschreibung

Allgemeine Beschreibung

Bromodomain testis-specific protein (BRDT), also known as Bromodomain-containing female sterile homeotic-like protein, or RING3-like protein, and encoded by the gene BRDT/ FSRG3 is a testis-specific chromatin protein that binds specifically histone H4 acetylated at ′Lys-5′ and ′Lys-8′ (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Recent studies have shown that BRDT plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. Therefore, it is involved in the late pachytene spermatocytes development. In the post-meiotic phase of spermatogenesis, BRDT binds to hyperacetylated histones and participates in their general removal from DNA. In addition, BRDT acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids, and participates in 3′-UTR truncation of specific mRNAs in post-meiotic spermatids. BRDT is required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. BRDT is testis-specific, and expressed in the germinal cells: in the early meiotic (pachytene) spermatocytes during spermiogenesis, in the round and elongating spermatids , and in the condensed late spermatids.

Immunogen

GST-tagged recombinant protein corresponding to mouse BRDT.

Anwendung

This Anti-BRDT antibody is validated for use in Immunohistochemistry for the detection of BRDT.

Qualität

Evaluated by Immunohistochemistry in mouse testis tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected BRDT in mouse testis tissue.

Zielbeschreibung

107 kDa calculated

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable