8.55015

Amino PEGA resin

Novabiochem^®

• Synonym(e): Amino PEGA resin
• CAS-Nummer: 372109-59-6
• UNSPSC-Code: 51171641
• NACRES: NA.22

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: Novabiochem^®; PEGA
• Form: beads
• Eignung der Reaktion: reaction type: Fmoc solid-phase peptide synthesis
• Hersteller/Markenname: Novabiochem^®
• Anwendung(en): peptide synthesis
• Funktionelle Gruppe: amine
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

PEGA resins consist of dimethyl acrylamide and mono-2-acrylamidoprop-1-yl^{[2-aminoprop-1-yl]} polyethylene glycol cross-linked with bis 2-acrylamidoprop-1-yl polyethyleneglycol. These supports swell extensively in a wide range of solvents and are permeable to macromolecules up to 35 kD, making them ideal for the preparation of combinatorial libraries, affinity purification and on-resin enzyme assays. For applications of these resins in SPPS and SPOS see ^{[1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18]}.Note: PEGA resins are supplied swollen in ethanol, with 1g dry resin corresponding to approximately 15 mL of swollen resin. As the beads of PEGA resins become very sticky and easily damaged when shrunk or dried, they are best handled in a swollen state. To use, the appropriate amount of swollen resin should be weighed into a reaction vessel and any residual ethanol removed by copious washings with the appropriate solvent or solvent mixture. After peptide assembly, the resin should be washed with DCM and transferred to the cleavage vessel. Excess DCM can be removed under vacuum, and the shrunk resin treated with the TFA cocktail. The characteristics of the swollen resin, its robustness and high permeability enables its use in both batch and continuous flow synthesis apparatus.

Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references

Literature references

^[1] M. Meldal, et al. (1992) Tetrahedron Lett., 33, 3077.
^[2] F. I. Auzanneau, et al. (1994) J. Peptide Sci., 1, 31.
^[3] M. Meldal in ′Peptides 1992, Proc. 22nd European Peptide Symposium′, C. H. Schneider & A. N. Eberle (Eds), ESCOM, Leiden, 1993, pp.61.
^[4] M. Meldal, et al. in ′Innovation & Perspectives in Solid Phase Synthesis, 3rd International Symposium′, R. Epton (Eds), Mayflower Worldwide, Birmingham, 1994, pp. 259.
^[5] M. Meldal, et al. (1993) Int. J. Peptide Protein Res., 41, 250.
^[6] M. Meldal, et al. (1994) J. Chem. Soc., Chem. Commun., 849.
^[7] M. Meldal, et al. (1994) Proc. Natl. Acad. Sci. USA, 91, 3314.
^[8] M. Renil, et al. (1998) J. Peptide Sci., 4, 195.
^[9] J. C. Spetzler, et al. (1998) J. Peptide Sci., 4,128.
^[10] M. Meldal, et al. (1998) J. Peptide Sci., 4 ,83.
^[11] J. A. Camarero, et al. (1998) J. Peptide Res., 51, 303.
^[12] J. A. Camarero, et al. (2000) Lett. Pept. Sci., 7,17.
^[13] O. Melnyk, et al. (2001) J. Org. Chem., 66, 4153.
^[14] J. Buchardt, et al. (2000) J. Comb. Chem., 2, 624.
^[15] J. F. Tolberg, et al. (2002) J. Org. Chem., 67, 4143.
^[16] J. Bondebjerg, et al. (2002) J. Am. Chem. Soc., 124, 11046.
^[17] J. Tulla-Puche, et al. (2004) J. Org. Chem., 69, 4101.
^[18] S. Aggarwal, et al. (2005) Biomaterials, 26, 6077.

Anwendung

• Fluorescence-Based On-Resin Detection of Three Model Proteases: Discusses the use of Amino PEGA resin modified with backbone amide linker (BAL) for developing solid support-bound probes used in fluorescence-based assays. (Milicevic & Hlavác, 2021).
• Attachment of cyclodextrin acids to PEGA resin and study of binding with fluorescence microscopy: Investigates the attachment of cyclodextrin to amino PEGA resin and evaluates their binding interactions using fluorescence microscopy. (Langhorn et al., 2021).

Verlinkung

Replaces: 01-64-0100

Hinweis zur Analyse

Farbe (visuell): weiß bis gelb bis beige
Aussehen der Substanz (visuell): Kügelchen
Beladung (Photometrische Bestimmung des Fmoc-Chromophores nach Abspaltung vom Fmoc-Leu beladenen Harz.): 0.30 - 0.50 mmol/g
Aufquellvolumen (in DMF): chargenspezifischer Wert
Aufquellvolumen (in Wasser): chargenspezifischer Wert
Trockenanteil %: chargenspezifischer Wert
Das Harz wird in Ethanol gequollen verkauft. 50 - 100 mesh

Rechtliche Hinweise

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable