8.51025

5-Carboxyfluorescein

Novabiochem^®

• Synonym(e): 5-FAM
• Empirische Formel (Hill-System): C₂₁H₁₂O₇
• CAS-Nummer: 76823-03-5
• Molekulargewicht: 376.32
• MDL-Nummer: MFCD00036874
• UNSPSC-Code: 12352005
• NACRES: NA.22

Eigenschaften

• Qualitätsniveau: 200
• Produktlinie: Novabiochem^®
• Assay: ≥98% (TLC); ≥99.0% (HPLC)
• Form: powder
• Eignung der Reaktion: reagent type: fluorescent-labelling reagent
• Hersteller/Markenname: Novabiochem^®
• Anwendung(en): peptide synthesis
• Lagertemp.: 2-8°C
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChIKey: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Anwendung

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Verlinkung

Replaces: 01-63-0112

Hinweis zur Analyse

Farbe (visuell): gelb bis orange
Aussehen der Substanz (visuell): Pulver
Identität (IR): entspricht
Reinheit (DC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Gehalt (HPLC, Fl.%): ≥ 99.0 % (a/a)
DC-Bedingungen gemäß den Angaben unseres Hauptkataloges

Rechtliche Hinweise

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable