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Merck

70953

Sigma-Aldrich

Rosetta Competent Cells - Novagen

Escherichia coli, rod shaped

Synonym(e):

Competent cells

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About This Item

UNSPSC-Code:
41106202
NACRES:
NA.81

product name

Rosetta Competent Cells - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Biologische Quelle

Escherichia coli

Qualitätsniveau

Hersteller/Markenname

Novagen®

Lagerbedingungen

OK to freeze

Wachstumsmodus

adherent or suspension

Morphologie

rod shaped

Methode(n)

microbiological culture: suitable

Zelltransformation

transformation efficiency: >2×106 cfu/μg

Versandbedingung

dry ice

Lagertemp.

−70°C

Allgemeine Beschreibung

Genotype: F-ompT hsdSB(rB- mB-) gal dcm pRARE (CamR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Komponenten

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Warnhinweis

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Rechtliche Hinweise

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2


Analysenzertifikate (COA)

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mRNA modifications play important roles in regulating gene expression. One of the most abundant mRNA modifications is N6,2-O-dimethyladenosine (m6Am). Here, we demonstrate that m6Am is an evolutionarily conserved mRNA modification mediated by the Phosphorylated CTD Interacting Factor 1 (PCIF1), which
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BioTechniques, 58(2), 81-84 (2015-02-06)
Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target. One potential source of amplification bias is the inherent lack of codon optimization that occurs within phage
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Biocatalytic transformations generally rely on purified enzymes or whole cells to perform complex transformations that are used on industrial scale for chemical, drug, and biofuel synthesis, pesticide decontamination, and water purification. However, both of these systems have inherent disadvantages related

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