574597-M

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

liquid, Calbiochem^®

• Synonym(e): Anti-SOD
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: sheep
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: mouse, rat, human, monkey
• Hersteller/Markenname: Calbiochem^®
• Verfügbarkeit: not available in Canada,
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.

Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.

Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O₂-) to molecular oxygen (O₂) and hydrogen peroxide (H₂O₂).

This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

Immunogen

Human

highly purified, human erythrocyte SOD Cu/Zn.

Anwendung

Immunoblotting (1:1000)

Immunoelectrophoresis (see comments)

Frozen sections (see comments)

Paraffin sections (see comments)

Free-floating sections (see comments)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In glycine-buffered saline, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
Human red blood cell lysate

Sonstige Hinweise

Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.

This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable