567693

SOST, Mouse, Recombinant

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Assay: ≥90% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: <1.0 EU/μg protein Endotoxin
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.

Biochem./physiol. Wirkung

Inhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from 0.2 µm filter-sterilized PBS, BSA.

Rekonstituierung

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Sonstige Hinweise

Kusu, N. et al. 2003. J. Biol. Chem.278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet.10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet.68, 577.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3