539660

PKCε, Human, Recombinant, S. frugiperda

• Synonym(e): Protein Kinase Cε
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Assay: ≥85% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: liquid
• Spezifische Aktivität: ≥800 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Full-length, recombinant, human, PKCε expressed in S. frugiperda insect cells using a baculovirus expression system. Suitable for studies of PKCε and its regulation. PKCε is a Ca²⁺-independent and phospholipid-dependent enzyme.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC_ε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.4.

Physikalische Form

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Newton, A.C. 1995. J. Biol. Chem.270, 28495.
Dekker, L.V., and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M., and Burns, D.J. 1991. J. Biol. Chem.266, 4661.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable