527460

PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

liquid, Calbiochem^®

• Synonym(e): Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human, mouse
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG
• Versandbedingung: wet ice
• Lagertemp.: −70°C
• Posttranslationale Modifikation Target: phosphorylation (pThr451)
• Angaben zum Gen: human ... EIF2AK2(5610)

Beschreibung

Allgemeine Beschreibung

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

Anwendung

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In Dulbecco′s PBS (without Mg⁺² or Ca⁺²), 0.1% BSA, 50% glycerol, pH 7.3.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Hinweis zur Analyse

Positive Control
IFNγ-stimulated HeLa cells.

Sonstige Hinweise

Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.

When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable