496260

Oncostatin M, Human, Recombinant, E. coli

• Synonym(e): Oncostatin M, Human, Recombinant, E. coli
• CAS-Nummer: 106956-32-5
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 100
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: ≤1 EU/μg Endotoxin (EU/μg rhOSM)
• Versandbedingung: ambient
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Enhances the growth of both endothelial and hematopoietic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposis sarcoma cells, and a human erythroleukemic cell line, TF-1.

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposi′s sarcoma cells, and human erythroleukemia cell line, TF-1.

Biochem./physiol. Wirkung

ED₅₀ = 0.15-0.30 ng/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg rhOSM.

Rekonstituierung

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% BSA or HSA.

Sonstige Hinweise

Boileau, C., et al. 2000. J. Immunol.164, 5713.
Miyajima, A., et al. 2000. Cytokine Growth Factor Rev.11, 177.
Linsley, P.S., et al. 1990. Mol. Cell. Biol.10, 1882.
Kitamura, T., et al. 1989. J. Cell Physiol.140, 323.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3