482728
Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb
liquid, Calbiochem®
Synonym(e):
Anti-iNOS
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität
mouse, rat, human
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
avoid repeated freeze/thaw cycles
Isotyp
IgG
Versandbedingung
wet ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... NOS1(4842)
Allgemeine Beschreibung
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
Recognizes the ~130 kDa iNOS protein.
This Anti-Nitric Oxide Synthase, Inducible Rabbit pAb is validated for use in Frozen Sections, WB, ICC, Paraffin Sections for the detection of Nitric Oxide Synthase, Inducible.
Immunogen
Mouse
a synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
Anwendung
Frozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
Undiluted serum.
Rekonstituierung
Following initial thaw, aliquot and freeze (-20°C).
Hinweis zur Analyse
Positive Control
Raw 264.7 cells treated with IFNγ and LPS
Raw 264.7 cells treated with IFNγ and LPS
Sonstige Hinweise
Exhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.
Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Produkt-Auswahlhilfe.
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Jae Hwan Kim et al.
Experimental neurobiology, 26(6), 380-389 (2018-01-06)
Ischemic preconditioning (IP) is one of the most important endogenous mechanisms that protect the cells against ischemia-reperfusion (I/R) injury. However, the exact molecular mechanisms remain unclear. In this study, we showed that changes in the level of agmatine were correlated
Dejia Li et al.
PloS one, 6(6), e21618-e21618 (2011-07-09)
Nitrosative stress compromises force generation in Duchenne muscular dystrophy (DMD). Both inducible nitric oxide synthase (iNOS) and delocalized neuronal NOS (nNOS) have been implicated. We recently demonstrated that genetic elimination of nNOS significantly enhanced specific muscle forces of the extensor
David L Schreibman et al.
Neurocritical care, 29(2), 253-263 (2018-04-28)
Spontaneous intracerebral hemorrhage (ICH) leaves most survivors dependent at follow-up. The importance of promoting M2-like microglial responses is increasingly recognized as a key element to ameliorate brain injury following ICH. The osmotherapeutic agents, mannitol and hypertonic saline (HTS), which are
Chanyong Yun et al.
Mediators of inflammation, 2016, 8027537-8027537 (2016-09-21)
The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and
S Nuedling et al.
FEBS letters, 502(3), 103-108 (2001-10-05)
Physiological effects of estrogen on myocardium are mediated by two intracellular estrogen receptors, ERalpha and ERbeta, that regulate transcription of target genes through binding to specific DNA target sequences. To define the role of ERbeta in the transcriptional activation of
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.