407711

Interleukin-12, Human, Recombinant, S. frugiperda

• Synonym(e): rhIL-12
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Assay: ≥95% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: lyophilized
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: ≤1.0 EU/μg Endotoxin (EU/μg IL-12)
• Versandbedingung: ambient
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B cells, and connective tissue-type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T cell differentiation and function.

Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B-cells, and connective tissue type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T-cell differentiation and function.

Biochem./physiol. Wirkung

ED₅₀ = 50-200 pg/ml as measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-12.

Rekonstituierung

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Sonstige Hinweise

Kang, K., et al. 1996. J. Immunol. 156, 1402.
Riemann, H., et al. 1996. J. Immunol. 156, 1799.
Brunda, M.J., et al. 1994. J. Leukoc. Biol. 55, 280.
Smith, T.J., et al. 1994. Eur. J. Immunol. 24, 822.
Gubler, U., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 4143.
Stern, A.S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 6808.
Kobayashi, M., et al. 1989. J. Exp. Med. 170, 827.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable