368059-M

Granzyme B Substrate II, Fluorogenic

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity.

• Synonym(e): Granzyme B Substrate II, Fluorogenic, Z-IETD-AFC, Caspase-8 Substrate II
• Empirische Formel (Hill-System): C₃₇H₄₂F₃N₅O₁₃
• Molekulargewicht: 821.75
• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥97% (HPLC)
• Form: lyophilized solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: white
• Löslichkeit: DMSO: 1 mg/mL
• Fluoreszenz: λ_ex ~400 nm; λ_em ~505 nm
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for the detection of granzyme B. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Biochem./physiol. Wirkung

Primary Target
Fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

Z-Ile-Glu-Thr-Asp-AFC

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sonstige Hinweise

Karahashi, H., and Amano, F. 2000. Biol. Pharm. Bull. 23, 140.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable