218765

Caspase-9 Substrate I, Fluorogenic

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

• Synonym(e): Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC
• Empirische Formel (Hill-System): C₃₃H₃₈F₃N₇O₁₁
• CAS-Nummer: 210345-03-2
• Molekulargewicht: 765.69
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; desiccated; protect from light
• Farbe: white
• Löslichkeit: DMSO: 5 mg/mL
• Fluoreszenz: λ_ex ~400 nm; λ_em ~505 nm
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Biochem./physiol. Wirkung

Cell permeable: no

Primary Target
A fluorogenic substrate for caspase-9

Product does not compete with ATP.

Reversible: no

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

Ac-Leu-Glu-His-Asp-AFC

Physikalische Form

Supplied as a trifluoroacetate salt.

Rekonstituierung

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sonstige Hinweise

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable