208694

Calmodulin, Rinderhirn, hohe Reinheit

Calmodulin is a ubiquitous Ca²⁺-binding protein that serves as a physiological effector of a wide range of biological processes. Purified from bovine brain.

• Synonym(e): Calmodulin, Rinderhirn, hohe Reinheit, CaM, Ca²⁺/Calmodulin
• CAS-Nummer: 73298-54-1
• MDL-Nummer: MFCD00131869
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: bovine brain
• Qualitätsniveau: 200
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized
• Spezifische Aktivität: ≥250,000 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Methode(n): cell based assay: suitable
• Löslichkeit: aqueous buffer: soluble; water: soluble
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: bovine ... Calm(32659)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Calmodulin (CaM) ranks among the most highly conserved protein sequences, with only histone proteins H4 and H3, actin B, and ubiquitin showing greater evolutionary conservation. CaM is a primarily helical protein with N- and C-terminal domains, each containing two paired Ca2+-binding EF-hand motifs. These motifs, with pentagonal-bipyramidal geometry, feature a conserved 12-amino acid sequence (positions 1–12) within a canonical helix-loop-helix structure.

Anwendung

Calmodulin, Bovine Brain, High Purity has been used to study melatonin-calmodulin interaction and to activate Calcium–calmodulin (CaM)-dependent protein kinase IIa (CaMKII)a through in vitro incubation.

Biochem./physiol. Wirkung

Calmodulin (CaM) is a ubiquitous Ca²⁺-binding protein distributed in eukaryotic cells that serves as a major physiological effector for a wide range of cellular responses. The Ca²⁺-calmodulin complex is involved in the maintenance of intracellular Ca²⁺ homeostasis, as well as in the regulation of the intracellular concentration of other second messengers, such as cyclic AMP and inositol trisphosphate. Mutations in the CaM sequence can result in diverse and potentially life-threatening pathologies such as ventricular tachycardia, congenital arrhythmia, and long QT syndrome. These mutations have the capacity to modify CaM′s ability to bind to Ca²⁺ and its target proteins and peptides, thereby disrupting their subsequent activity. Ca²⁺/CaM complex and CaM-binding domains (CaMBDs) interactions influence memory formation, muscle contraction, cellular metabolism, and cytoskeletal rearrangements.

Verpackung

Ja

Warnhinweis

Toxizität: Standard-Handhabung (A)

Einheitendefinition

Eine Einheit ist als die Menge Calmodulin definiert, die 50 % der maximalen Enzymaktivierung eines Standardwerts von Aktivator-defizientem Calcineurin auslöst.

Physikalische Form

Aus 30 mmol/l HEPES, 1 mmol/l CaCl₂, 0,1 mmol/l DTT, pH-Wert 7,4, lyophilisiert.

Rekonstituierung

Das chargenspezifische Rekonstitutionsvolumen entnehmen Sie bitte dem Fläschchenetikett.

Nach der Rekonstitution in Aliquote aufteilen und einfrieren (-20 °C bzw. -70 °C). Wässrige Stammlösungen sind bei einer Temperatur von -20 °C bis zu 2 Monate und bei einer Temperatur von -70 °C bis zu 4 Monate haltbar.

Sonstige Hinweise

This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3