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Merck

1.52321

Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 50 mm × 2 mm HPLC Column

Synonym(e):

Chromolith® HighResolution RP-18 endcapped 50-2 HPLC column

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About This Item

UNSPSC-Code:
41115709
NACRES:
SB.52

product name

Chromolith® HighResolution RP-18 endcapped, L × I.D. 50 mm × 2 mm HPLC column

Qualitätsniveau

Leistungsmerkmale

endcapped

Kennzeichnungsgrad

18% Carbon loading

Parameter

200 bar pressure
50 °C max. temp.

Methode(n)

HPLC: suitable
mass spectrometry (MS): suitable (High)

Säule L × ID

50 mm × 2 mm

Oberflächenbereich

250 m2/g

Matrix

highly porous
monolithic silica

Aktive Matrixgruppe

C18 bonding phase, octadecylsilane (ODS)

Porengröße

1.5 μm macroporosity
15 nm mesoporosity

operating pH range

2-7.5

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Allgemeine Beschreibung

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), and the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for fast and high-resolution separations meeting UHPLC and LC-MS needs.

Chromolith® HighResolution RP-18 endcapped columns with 2 mm internal diameter are ideal for use with UHPLC or UPLC instruments, thanks to their very small internal volumes. A particular benefit is very fast analysis, reduced sample preparation and the low column backpressure. Ultra-high performance and extremely low operating pressure make Chromolith® 2 mm I.D. columns truly unique. Chromolith® and Chromolith® HighResolution columns are perfectly suitable for LC-MS use; however, the column dimension of 2 mm I.D. or below is preferred to be used in LC-MS methods.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52325] with the Chromolith® Guard Cartridge Holder 5 mm x 2 mm [1.52355]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Rechtliche Hinweise

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysenzertifikate (COA)

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Artikel

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

HPLC-DAD workflow analyzes 14 cannabinoids in hemp extracts in 10 minutes using Chromolith columns for robust performance.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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