14-930-M

IRE1, active

• UNSPSC-Code: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Mol-Gew.: M_w 62 kDa
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• NCBI-Hinterlegungsnummer: NM_001433
• UniProt-Hinterlegungsnummer: O75460

Beschreibung

Allgemeine Beschreibung

Product Source: N-terminal 6His-tagged, recombinant, human IRE1 amino acids 465-end expressed by baculovirus in Sf21 insect cells.

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Verpackung

Also available in 250μg size as catalog # 14-930M and in bulk as catalog # 14-930-K.

Qualität

Routinely evaluated by phosphorylation of MBP.

Physikalische Form

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Lagerung und Haltbarkeit

6 months at -70°C

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable