14-782-M

PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.

• Synonym(e): Active Kinase Protein, Human PI 3-Kinase
• UNSPSC-Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100; 300
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Mol-Gew.: M_w 125.3 kDa (p110α; E542K); M_w 83.7 kDa (p85α)
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• NCBI-Hinterlegungsnummer: NM_006218.2
• UniProt-Hinterlegungsnummer: P42336
• Angaben zum Gen: human ... PIK3CA(5290)

Beschreibung

Allgemeine Beschreibung

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K and untagged, recombinant, full-length human p85α.

The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.

Anwendung

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.

Verpackung

Also available in 250 μg size --call for pricing and availability and reference catalog number 14-782M when ordering the 250 μg size.

Lagerung und Haltbarkeit

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable