07-1519

Anti-phospho Grb10 (Ser503) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, CD252
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse
• Speziesreaktivität (Voraussage durch Homologie): primate (based on 100% sequence homology), porcine (based on 100% sequence homology), human (immunogen homology)
• Methode(n): inhibition assay: suitable (peptide); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005302
• UniProt-Hinterlegungsnummer: Q13322
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer503)
• Angaben zum Gen: human ... GRB10(2887) ; mouse ... Grb10(14783) ; pig ... Grb10(100188977)

Beschreibung

Allgemeine Beschreibung

Grb10 (insulin receptor-binding protein Grb-IR) is a ubiquitous adapter protein which is expressed in both fetal and adult tissues. It is a multidomain protein that interacts with a wide array of cellular targets; the PH domain allows interaction with several subtypes of phosphoinositides; the Ras-associating domain binds a wide variety of Ras GTPases; and the SH domain facilitates binding to membrane-bound tyrosine kinase receptors which link Grb10 to different signaling pathways. Most notably, Grb10 associates with the insulin receptor at phosphotyrosine residues via its C-terminal BPS (between PH and SH) domain and inhibits the enzymatic activity of the insulin receptor. Grb10 also acts downstream of the insulin receptor pathway to inhibit activation of AKT-1. In addition, Grb10 suppresses Wnt signalling by binding to the low-density lipoprotein receptor. In fetal tissues, Grb10 may inhibit growth and placental size.

Spezifität

Other homologies: Rat (91% sequence homology).

This antibody recognizes Grb10 phosphorylated at (Ser503).

Immunogen

Epitope: Phosphorylated (Ser503)

KLH-conjugated linear peptide corresponding to human GRB10 phosphorylated at (Ser503).

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Entwicklungsabhängige Signalübertragung

Peptide Inhibition Assay Analysis: 1 µg/mL from a representative lot detected phospho-Grb10 (Ser503) on 10 µg of embryonic fibroblast cell lysate + non-phospho-Grb10 (Ser503) peptide. However, the antibody was inhibited when incubated with a phospho-Grb10 (Ser503) peptide.

This Anti-phospho Grb10 (Ser503) Antibody is validated for use in Peptide Inhibition Assay, Western Blotting for the detection of phospho Grb10 (Ser503).

Qualität

Evaluated by Western Blot in mouse embryonic fibroblast cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-Grb10 (Ser503) on 10 µg of mouse embryonic fibroblast cell lysate.

Zielbeschreibung

~75 kDa observed. An uncharacterized band appears at ~16 kDa in some lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Mouse embryonic fibroblast cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable