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06-157

Sigma-Aldrich

Anti-RasGAP Antibody

Upstate®, from rabbit

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Speziesreaktivität

human, rat, mouse, avian

Hersteller/Markenname

Upstate®

Methode(n)

immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... TSR3(115939)

Spezifität

GAP

Immunogen

Trp E fusion corresponding to residues 171-448 of human RasGAP

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
MAP-Kinasen
Anti-RasGAP Antibody is an antibody against RasGAP for use in IP & WB.

Qualität

Western Immunoblot Analysis: This antibody, at 0.5 and 2.0 μg/ml, recognized the 120 kDa GAP protein in a RIPA lysate of human Raji lymphocyte cells, lysates of rat L6 myoblasts and mouse Wehi leukemia cells.
Immunoprecipitation: 5 μg of this antibody immunoprecipitated the 120kDa GAP protein from a RIPA lysate of human Raji lymphocyte cells.

Zielbeschreibung

120kDa

Verlinkung

Replaces: 04-577; 04-1039

Physikalische Form

Protein A chromatography
200 μg of protein-A purified IgG. Lyophilized from 200 μl of 0.1 M Tris-HCl, 0.15 M NaCl, and 0.05% Na Azide.
Format: Purified

Lagerung und Haltbarkeit

Lyophilized: 2 years at -20°C; Rehydrated: 6 months at -20°C Rehydrate in 200 μl of water.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

Exclamation mark

Signalwort

Warning

Gefahreneinstufungen

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

S J Ruff et al.
The Journal of biological chemistry, 272(2), 1263-1267 (1997-01-10)
The intraperitoneal injection of a vanadate/H2O2 mixture (peroxovanadate) into mice resulted within minutes in the appearance of numerous tyrosine-phosphorylated proteins in the liver and kidney. These effects are presumably due to the inhibition of phosphotyrosine phosphatase activity. Three of the
F Authier et al.
The Biochemical journal, 319 ( Pt 1), 149-158 (1996-10-01)
We have investigated the biosynthesis, subcellular location and expression of insulin-degrading enzyme (IDE). a type-I peroxisomal protease, in semi-permeabilized hepatoma cells using pulse-chase experiments, non-denaturing immunoprecipitation protocols and Northern-blot analyses. In HcpG2 cell lysates prepared from cells radiolabelled with Tran[35S]-label
A conserved region of c-Ha-Ras is required for efficient GTPase stimulation by GTPase activating protein but not neurofibromin
Yoder-Hill, J., et al
The Journal of Biological Chemistry, 270, 27615-27621 (1995)
S J Taylor et al.
The Journal of biological chemistry, 270(17), 10120-10124 (1995-04-28)
The c-Src tyrosine kinase phosphorylates and binds to a 68-kDa RNA-binding protein in mitotic cells. We have examined the mechanism and functional consequence of the interaction of c-Src with this protein, Sam 68 (Src associated in mitosis, 68 kDa). In
P J Owen-Lynch et al.
The Journal of biological chemistry, 270(11), 5956-5962 (1995-03-17)
A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)-dependent IC.DP cells grown at the permissive temperature (32 degrees C) but not at the restrictive temperature (39 degrees C).

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