05-488-I

Anti-Pyk2/CAKβ Antibody

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 78, monoclonal
• Mol-Gew.: calculated mol wt 115.78 kDa; observed mol wt ~110 kDa
• Speziesreaktivität: human, rat
• Verpackung: antibody small pack of 100 μg
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P70600
• Versandbedingung: dry ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rat ... Ptk2b(50646)

Beschreibung

Allgemeine Beschreibung

Protein-tyrosine kinase 2β (UniProt: P70600; also known as EC:2.7.10.2, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Pyk2) is encoded by the Ptk2b (also known as Pak2, Pyk2) gene (Gene ID: 50646) in rat. Pky2 is a non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. Its protein kinase domain is localized in amino acids 425-683. Pyk2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It is highly expressed in pulmonary vein endothelial cells, lung, and brain. It localizes to the cytoplasmic side of the membrane. Pyk2 is activated by phosphorylation of tyrosine residues in response to various stimuli that elevate intracellular calcium levels. Tyr402 is the major autophosphorylation site, but it can also be phosphorylated by other kinases. This phosphorylation promotes interaction with Src and Src family members, leading to phosphorylations at Tyr579, Tyr580, and Tyr881. Phosphorylation at Tyr402, Tyr579 and Tyr580 is required for its optimal kinase activity and phosphorylation at Tyr881 is essential for its interaction with Grb2.

Spezifität

Clone 74 is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2β (Pyk2). It targets an epitope within the N-terminal half.

Immunogen

GST-tagged recombinant fragment corresponding to 412 amino acids from the N-terminal half of rat Protein-tyrosine kinase 2-beta (Pyk2).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.

Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).

Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.

Physikalische Form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at +2°C to +8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable