05-347

Anti-PCNA Antibody, clone PC10

clone PC10, Upstate^®, from mouse

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: culture supernatant
• Antikörper-Produkttyp: primary antibodies
• Klon: PC10, monoclonal
• Speziesreaktivität: vertebrates
• Hersteller/Markenname: Upstate^®
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NM_002592
• UniProt-Hinterlegungsnummer: P12004
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PCNA(5111)

Beschreibung

Allgemeine Beschreibung

Expression of proliferating cell nuclear antigen (PCNA), cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Anti-PCNA reacts with PCNA at the molecular weight of 36 kDa on western blot.

Spezifität

PCNA

Immunogen

Recombinant rat PCNA

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Detect PCNA with Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC & IHC.

Qualität

routinely evaluated by immunoblot on RIPA lysate from human A431 cells or mouse 3T3/A31 cell lysate

Zielbeschreibung

36kDa

Physikalische Form

Concentrated culture supernatant

Format: Purified

Protein A purified mouse immunoglobulin in PBS containing 1 mg/mL BSA and 10mM Sodium Azide.

Lagerung und Haltbarkeit

2 years at -20°C

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable