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G005519

HPLC Analysis of Mephobarbital Enantiomers on Astec® Cellulose DMP

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Astec Cellulose DMP; 25 cm x 4.6 mm I.D., 5 μm particles (51099AST)
mobile phase: [A] heptane; [B] isopropanol; (80:20, A:B)
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 254 nm
injection: 15 μL
sample: mephobarbital, 1 g/L in 50:50, heptane:isopropanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

Mephobarbital enantiomers are resolved by normal phase chromatography.

Other Notes

Mephobarbital (aka methyphenobarbital) is used as a sedative, anxiolytic and anticonvulsant. It is the N-methylated analogue of phenobarbital, and has similiar indications, therapeutic value, and tolerability.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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Description
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mobile phase component

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standard

Product No.
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M3887

Mephobarbital

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