ZRB2349
Anti-ApoER2/LRP-8 Antibody, clone 1B13 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
LRP-8, Low-density lipoprotein receptor-related protein 8
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1B13, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 105.63 kDa
observed mol wt ~110 kDa
purified by
using Protein A
species reactivity
human
species reactivity (predicted by homology)
horse, monkey, feline, mouse, rat
packaging
antibody small pack of 25
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgGκ
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... LRP8(7804)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1B13 is a ZooMAb® rabbit recombinant monoclonal antibody that detects ApoER2. It targets an epitope within 18 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human ApoER2.
Application
Quality Control Testing
Evaluated by Western Blotting in U-87 MG cell lysate.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected ApoER2 in U-87 MG cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ApoER2/LRP-8 in HT-29 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ApoER2/LRP-8 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ApoER2/LRP-8 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ApoER2/LRP-8 in HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in U-87 MG cell lysate.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected ApoER2 in U-87 MG cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ApoER2/LRP-8 in HT-29 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ApoER2/LRP-8 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ApoER2/LRP-8 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ApoER2/LRP-8 in HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Low-density lipoprotein receptor-related protein 8 (UniProt: Q14114; also known as LRP-8, Apolipoprotein E receptor 2) is encoded by the LRP8 (also known as APOER2) gene (Gene ID: 7804) in human. ApoER2 is a member of the LDL receptor family, which is characterized by distinct structural motifs, including an N-terminal ligand-binding domain with multiple cysteine-rich repeats, EGF precursor homology domains containing YWTD β-propeller, an O-linked sugar domain near the membrane-spanning segment, and a cytoplasmic domain containing the NPXY motif. ApoER2 is involved in mediating cellular uptake of lipoprotein particles and is integral to signal transduction pathways influencing neuronal migration, synaptic plasticity, and cell signaling. It interacts with several ligands, including Apolipoprotein E, Reelin, and alpha-2-macroglobulin, among others. LRP8 is ubiquitously expressed but shows a higher expression level in certain tissues like the brain, where it plays a significant role in neurodevelopment and synaptic functions. Its expression can be regulated at both the transcriptional and post-transcriptional levels, influenced by cellular conditions and developmental stages. ApoER2 has been implicated in several diseases, including Alzheimer′s disease, atherosclerosis, and cancer. Its dysregulation has been associated with altered lipid metabolism, impaired synaptic function, and abnormal cellular proliferation and migration.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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