W0138
Western Blocker™ Solution
for HRP detection systems
Synonym(s):
Western blot blocking solution, blocking buffer
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| Size/SKU | Availability | Price |
|---|---|---|
400 mL | Please contact Customer Service for Availability | CZK 3,000.00 |
About This Item
NACRES:
NA.25
UNSPSC Code:
41105329
CZK 3,000.00
Please contact Customer Service for Availability
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes.[1][2] It has also been used for dilution of antibodies in western blot.[3]
Western Blocker Solution has been used for blocking of western blot membranes.[1][2] It has also been used for dilution of antibodies in western blot.[3]
Features and Benefits
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
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| Quality Level 200 | Quality Level - | Quality Level 300 | Quality Level - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. - |
| shelf life ≥2 yr | shelf life - | shelf life - | shelf life - |
![Western Blocking Reagent, Solution solution, sufficient for 10 blots (11921673001 [100 cm2]), sufficient for 60 blots (11921681001 [100 cm2])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Hazard Classifications
ED ENV 1
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Danger
hcodes
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
9002-93-1
CAS No.
9002-93-1
CAS No.
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