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V4014

Anti-V5-Cy3 antibody, Mouse monoclonal

purified immunoglobulin, ~1 mg/mL, clone V5-10

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Synonym(s):

Monoclonal Anti-V5

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Size/SKUAvailabilityPrice
100 μg
Please contact Customer Service for Availability
12 000,00 CZK

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
Conjugate:
CY3 conjugate
Clone:
V5-10, monoclonal
Application:
ICC
Citations:
6

12 000,00 CZK

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biological source

mouse

Quality Segment

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 2.0-4.0 μg/mL

shipped in

wet ice

storage temp.

2-8°C

General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

Application

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy3 is a trademark of Cytiva

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This Item
V8012V2260C0992
description

purified immunoglobulin, ~1 mg/mL, clone V5-10

description

clone V5-10, purified from hybridoma cell culture

description

clone V5-10, purified immunoglobulin, lyophilized powder

description

clone CY-96, purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

conjugate

CY3 conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

CY-96, monoclonal

technique(s)

immunocytochemistry: 2.0-4.0 μg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

technique(s)

western blot: 1:4,000-1:8,000

technique(s)

immunocytochemistry: suitable, dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5, microarray: suitable, immunoprecipitation (IP): suitable, direct ELISA: suitable

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C


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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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