The reconstituted urease solution is stable for at least one month when stored at 2–8 °C. Stability may be further extended by freezing. The stability is not effected following multiple freeze–thaw cycles. When reconstituted in 30 mL water, the solution will contain 100 units urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
U3383
Urease Buffer Reagent
Synonym(s):
Urea colorimetric determination assay reagent
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Please contact Customer Service for Availability | 3 380,00 CZK |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
3 380,00 CZK
Please contact Customer Service for Availability
description
When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Quality Segment
form
powder
mol wt
545 kDa
storage temp.
2-8°C
General description
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.
Application
Urease Buffer Reagent has been used:
- to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
- in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
- to study the metabolic capacity of rat hepatocytes
Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.
1 of 1
This Item | |||
|---|---|---|---|
| description - | description BioUltra, >0.1 M Na2CO3, >0.2 M NaHCO3 | description BioUltra, 1 M in H2O | description - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form powder | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. 2-8°C |
| description When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative. | description - | description - | description - |
| mol wt 545 kDa | mol wt - | mol wt - | mol wt - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class
13 - Non Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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How long can we keep the solution after reconstitution? What is the recommended storage condition after reconstitution?
1 answer-
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What's the concentration of Jack bean Urease? I can only see the concentration of buffer.
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As provided, this product contains 100 units per vial. The mass weight per vial is not determined.
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How many urease units does 1 vial contain?
1 answer-
As provided, this product contains 100 units per vial.
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What is the volume of water used for reconstitution of 1 vial?
1 answer-
As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.
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