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U0880

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Uricase from Candida sp.

Synonym(s):

Urate: oxygen oxoreductase

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Size/SKUAvailabilityPrice
250 units
Please contact Customer Service for Availability
CZK 6,220.00
1000 units
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CZK 12,000.00

About This Item

CAS Number:
9002-12-4
UNSPSC Code:
12352204
PubChem Substance ID:
24900619
NACRES:
NA.54
EC Number:
1.7.3.3 (BRENDA, IUBMB)
MDL number:
MFCD00082126
Specific activity:
≥2 units/mg solid
Recombinant:
expressed in E. coli

CZK 6,220.00

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recombinant

expressed in E. coli

Quality Segment

200

form

lyophilized powder

specific activity

≥2 units/mg solid

mol wt

35 kDa by SDS-PAGE

storage temp.

−20°C

SMILES string

[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment. [1]
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem/physiol Actions

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Physical form

Powder; contains citrate and sucrose

Other Notes

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

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Uricase from Candida sp. recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Sigma-Aldrich

U0880

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Uricase from Arthrobacter globiformis lyophilized powder, 15-30 units/mg protein (biuret)

Sigma-Aldrich

U7128

Uricase from Arthrobacter globiformis

Urease from Canavalia ensiformis (Jack bean) Type III, powder, 15,000-50,000 units/g solid

Sigma-Aldrich

U1500

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) powder, white, ~8 U/mg

Sigma-Aldrich

94281

Urease from Canavalia ensiformis (Jack bean)

specific activity

≥2 units/mg solid

specific activity

15-30 units/mg protein (biuret)

specific activity

15,000-50,000 units/g solid

specific activity

~8 U/mg

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized powder

form

lyophilized powder

form

powder

form

powder

mol wt

35 kDa by SDS-PAGE

mol wt

tetramer ~32 kDa

mol wt

~544620 Da

mol wt

Mr ~480000

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

100

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–2 of 2 Questions  

  1. How can a solution in unit/mL be prepared using product number U0880, with a pack size of 250 units and SKU U0880-250UN, considering that the uricase activity is greater than 2 units/mg solid? What is the procedure for preparing a 10 unit/mL solution from this?

    1 answer

    1. You can use the calculator provided at https://www.physiologyweb.com/calculators/units_per_volume_solution_concentration_calculator.html

      Based on a specific activity of 5 units/mg, the 250 unit pack size is equivalent to 50 mg in mass. To achieve a 10 units/mL solution from this, the 50 mg would need to be dissolved in 25 mL of solvent (e.g. PBS).

      Helpful?

  2. What is the amino acid sequence of this enzyme?

    1 answer

    1. The sequence is proprietary.

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