T8694

TrkB/Fc Chimera human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• UNSPSC Code: 51111800
• NACRES: NA.32
• MDL number: MFCD03458837
• Form: lyophilized powder
• Assay: >90% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in NSO cells
• Mol wt: 120-130 kDa by SDS-PAGE (reducing), calculated mol wt 71 kDa

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.1-0.4 μg/mL ED₅₀
• mol wt: 120-130 kDa by SDS-PAGE (reducing), calculated mol wt 71 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): western blot: suitable
• impurities: endotoxin, tested
• UniProt accession no.: Q16620
• storage temp.: −20°C
• Gene Information: human ... NTRK2(4915)

Description

Application

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis.[1] Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.[2]

Biochem/physiol Actions

Binds specifically to mediate neurotrophins BDNF and NT-4/5.

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.

TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.[3]

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Preparation Note

Reconstitute to 100 μg/mL in phosphate buffered saline.

Analysis Note

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

Other Notes

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable