T4904
Tris-Glycine Buffer 10× Concentrate
Synonym(s):
running buffer
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| Size/SKU | Availability | Price |
|---|---|---|
1 L | Please contact Customer Service for Availability | 5 770,00 CZK |
About This Item
UNSPSC Code:
41105323
EC Number:
232-650-8
NACRES:
NA.25
eCl@ss:
32110600
5 770,00 CZK
Please contact Customer Service for Availability
Application
Tris-Glycine buffer 10× concentrate has been used as a transfer buffer for Western blotting.
Biochem/physiol Actions
For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
Other Notes
0.25 M Tris, 1.92 M glycine, pH approx. 8.3
Contains no methanol.
Contains no methanol.
1 of 1
This Item | |||
|---|---|---|---|
| description - | description - | description pH 8.3, pHast Pack™, powder | description Protein-Concentrate Kit (Micro) is suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays & other applications. |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. - |
| sterility 0.2 μm filtered | sterility 0.2 μm filtered | sterility - | sterility - |
| foreign activity Protease, none detected | foreign activity - | foreign activity NICKase, none detected, Endonuclease , DNase, none detected, Exonuclease, none detected, Protease, none detected, RNase, none detected | foreign activity - |
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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