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T3516

Sigma-Aldrich

Thioflavin T

Powder

Synonym(s):

Basic Yellow 1, Thioflavine T

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5 G
CZK 1,940.00
25 G
CZK 3,750.00

CZK 1,940.00


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5 G
CZK 1,940.00
25 G
CZK 3,750.00

About This Item

Empirical Formula (Hill Notation):
C17H19ClN2S
CAS Number:
Molecular Weight:
318.86
Colour Index Number:
49005
Beilstein:
3922452
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

CZK 1,940.00


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Request a Bulk Order

Product Name

Thioflavin T, used as stain for amyloid

form

powder

composition

Dye content, ≥65%

solubility

methanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)-c2sc3cc(C)ccc3[n+]2C

InChI

1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

JADVWWSKYZXRGX-UHFFFAOYSA-M

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General description

Thioflavin T is a benzothiazole dye that increases in fluorescence upon binding to amyloid.[1][2] Thioflavin T has been used in histology and for protein characterization.[3]

Application

Thioflavin T has been used to identify the presence of amyloid fibrils in samples.[4]

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Tracking Aggregation and Fibrillation of Globular Proteins Using Terahertz and Far-Infrared Spectroscopies
Gretel Png
Taiwan yi Xue Hui za Zhi. Journal of the Formosan Medical Association (2016)
G A Cornwall et al.
Andrology, 7(5), 603-609 (2019-01-22)
We previously demonstrated the normal mouse epididymal lumen contains a non-pathological amyloid matrix that surrounds spermatozoa and plays important roles in sperm maturation and protection. The objective herein was to present a review of this work, including studies showing the
Jian Xiong et al.
Biophysical chemistry, 220, 42-48 (2016-11-20)
The hydrophobic fragment of the Alzheimer's related β-amyloid (Aβ) peptide, Aβ(25-40), aggregates and forms insoluble amyloid fibrils at a rate similar to the full-length peptide. In order to gain insight into the fibrillization of Aβ(25-40) and the ability of the
Extrinsic fluorescent dyes as tools for protein characterization.
Hawe, A., et al.
Pharmaceut. Res., 25, 1487-1499 (2008)
Pai Peng et al.
Nucleic acids research, 45(2), 541-546 (2016-12-03)
We present a conceptually new reversible nanosensor regulated by a DNA nanoswitch. This system is not only responsive to external stimuli (e.g. ATP) but also can be reversibly switched between 'OFF' and 'ON' states via toehold mediated strand displacement reactions.

Questions

1–2 of 2 Questions  
  1. How about the purity of this product?

    1 answer
    1. The purity of this material is indicated by the dye content. The minimum dye content of this product is 65%. A brief review of recent lots indicates that 85 - 90% dye content is typical. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/543/093/t3516pis.pdf

      Helpful?

  2. How do you reconsititute the powder?

    1 answer
    1. As described in the product data sheet, the product is soluble in methanol at 1mg/mL (0.1% w/v).
      Please see the product data sheet for additional solubility and handling recommendations: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/543/093/t3516pis.pdf

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