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Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Name: Triosephosphate Isomerase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:

9023-78-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-774-2

MDL number:

MFCD00082080

EC Number:

5.3.1.1 (BRENDA, IUBMB)

Specific activity:

~10,000 units/mg protein

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Properties

type

Type I

Quality Segment

200

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Description

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. [1] Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins. [2]

Physical form

Crystalline suspension in 2.7 M (NH₄)₂SO₄, 0.5 mM EDTA, pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

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This Item	P5381	T2391	P2621
Sigma-Aldrich T2507 Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich P5381 Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich T2391 Triosephosphate Isomerase from rabbit muscle	Sigma-Aldrich P2621 Phosphomannose Isomerase from Escherichia coli
specific activity ~10,000 units/mg protein	specific activity ≥400 units/mg protein (biuret)	specific activity ≥4,000 units/mg protein	specific activity ≥50 units/mg protein
form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension
mol wt 53 kDa by equilibrium centrifugation	mol wt tetramer 119,500 Da±600	mol wt calculated mol wt 53.2 kDa	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
foreign activity pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%	foreign activity -	foreign activity 3-phosphoglyceric phosphokinase ≤0.01%, glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%	foreign activity -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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