SRP2148
EF1α, GST tagged human
recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym(s):
CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, LENG7, PTI1, eEF1A-1
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10 μG
CZK 8,970.00
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10 μG
CZK 8,970.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.26
biological source
human
recombinant
expressed in E. coli
Assay
≥85% (SDS-PAGE)
form
frozen liquid
mol wt
~77.3 kDa
packaging
pkg of 10 μg
concentration
350 μg/mL
color
colorless to clear
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EEF1A1(1915)
Biochem/physiol Actions
The alpha subunit of elongation factor-1 (EEF1A) is involved in the binding of aminoacyl-tRNAs to 80S ribosomes. During the process, GTP is hydrolyzed into GDP. To perform this function, EEF1A has domains that bind guanine nucleotides, 80S ribosomes, and aminoacyl-tRNAs. Also, EEF1A interacts with the beta subunit of EEF1 to exchange bound GDP for GTP. Multimolecular complexes involving the eukaryotic elongation factor 1A (eEF1A) have been suggested to play an important role in the channeling (vectorial transfer) of tRNA during protein synthesis HIV-1 matrix protein (MA) was found to interact with elongation factor 1-α (EF1α), an essential component of the translation machinery that delivers aminoacyl-tRNA to ribosomes. The interaction between MA and EF1α impairs translation in vitro, a result consistent with a previously proposed model in which inhibition of translation by the accumulation of Gag serves to release viral RNA from polysomes, permitting the RNA to be packaged into nascent virions. It has been identified that the translation elongation factor, eEF1A, and its guanine nucleotide exchange factor, eEF1Bβ, as translationally controlled tumor protein (TCTP)-interacting partners.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Cans, C., et al.
Proceedings of the National Academy of Sciences of the USA, 24, 13892-13897 (2003)
J H Brands et al.
European journal of biochemistry, 155(1), 167-171 (1986-02-17)
The primary structure of the alpha subunit of elongation factor 1 (EF-1 alpha) from human MOLT 4 cells was determined by cDNA sequencing. The data show that the conservation of the amino acid sequence is more than 80% when compared
A Cimarelli et al.
Journal of virology, 73(7), 5388-5401 (1999-06-11)
Human immunodeficiency virus type 1 (HIV-1) gag-encoded proteins play key functions at almost all stages of the viral life cycle. Since these functions may require association with cellular factors, the HIV-1 matrix protein (MA) was used as bait in a
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