SRP2032

Topo I (C651-765) (C terminal domain) human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• Synonym(s): TOPI
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in insect cells
• Assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~15.2 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 500 μg/mL
• color: clear colorless
• NCBI accession no.: NM_003286
• UniProt accession no.: P11387
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... TOP1(7150)

Description

Biochem/physiol Actions

Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable