SRP0733

PCTK2 (CDK17)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: frozen liquid
• Assay: 70-95% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: ~64 kDa (Cyclin Y), ~88 kDa (PCTK2)

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 15-25 nmol/min·mg (radiometric)
• mol wt: ~64 kDa (Cyclin Y), ~88 kDa (PCTK2)
• concentration: 0.1 μg/μL
• accession no.: NM_002595, NM_145012
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CCNY(219771), CDK17(5128)

Description

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons ⁽¹⁾. The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins ⁽²⁾.

Preparation Note

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable