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SRP0393

Sigma-Aldrich

EZH2/SUZ12/RbAp48 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥88% (SDS-PAGE)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥88% (SDS-PAGE)

form

aqueous solution

mol wt

114 kDa (EZH2)
48 kDa (RbAP48)
87 kDa (SUZ12)

packaging

pkg of 20 μg

shipped in

dry ice

storage temp.

−70°C

Gene Information

General description

Complex of human EZH2 (GenBank Accession No. NM_004456) amino acids 2-end with N-terminal His-GST tag, MW= 114kDa; human SUZ12 (GenBank Accession No. NM_015355) amino acids 1-end with N-terminal His-tag, MW = 87kDa, and human RbAp48 (GenBank Accession No. NM_005610) amino acids 2-end with N-terminal His-tag, MW= 48 kDa, co-expressed in Baculovirus expression system.

Physical form

Formulated in 40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 18 mM Glutathione, 3mM DTT, and 20% glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Sooryanarayana Varambally et al.
Science (New York, N.Y.), 322(5908), 1695-1699 (2008-11-15)
Enhancer of zeste homolog 2 (EZH2) is a mammalian histone methyltransferase that contributes to the epigenetic silencing of target genes and regulates the survival and metastasis of cancer cells. EZH2 is overexpressed in aggressive solid tumors by mechanisms that remain
Rakotobe, D. et al.
Journal of Virology, 5, 32-32 (2008)

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