SRP0171

Jak2 Jh1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonym(s): JAK2, JTK10, Janus kinase 2, Just another kinase 2, Tyrosine-protein kinase JAK2
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: aqueous solution
• Assay: ≥80% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected insect cells
• Mol wt: 42.5 kDa

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 42.5 kDa
• packaging: pkg of 10 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_004972
• UniProt accession no.: O60674
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... JAK2(3717)

Description

General description

Human Jak2 (GenBank Accession No. NM_004972), (amino acids. 808-end) with N-terminal His tag, MW = 42.5 kDa, expressed in Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 1 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable