SML3403

HSip-1 DA trifluoroacetate hydrate

≥95% (HPLC)

• Synonym(s): Cyclen-AF-Cu DA trifluoroacetate hydrate, HSip 1 DA, HSip-1 diacetate trifluoroacetate hydrate, HSip1 DA, Hydrogen Sulfide imaging probe-1 DA, [N-(3′,6′-bis(Acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-5-yl)-1,4,7,10-tetraazacyclododecane-1-acetamide-κN1,κN4,κN7,κN10]-copper(2+) trifluoroacetate hydrate
• Linear Formula: C₃₄H₃₇CuN₅O₈· xCF₃COOH· yH₂O
• UNSPSC Code: 41106609
• NACRES: NA.77
• Assay: ≥95% (HPLC)
• Form: powder
• Storage condition: protect from light

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: powder
• mol wt: 707.23 g/mol
• storage condition: protect from light
• color: , Light Blue to Green Blue
• solubility: H₂O: 2 mg/mL, clear
• storage temp.: -10 to -25°C
• SMILES string: O=C1OC2(C3=CC=C(NC(C[N]45CCN6CCN7CCN([Cu@+2]765)CC4)=O)C=C13)C8=CC=C(C=C8OC9=CC(OC(C)=O)=CC=C92)OC(C)=O.[y H2O]

Description

Biochem/physiol Actions

Cell-permeable HSip-1 diacetate precursor. Sensitive and selective intracellular hydrogen sulfide (H2S) fluorescent probe.

HSip-1 DA corresponds to the cell-permeable diacetate ester precursor of the sensitive hydrogen sulfide (H2S)-selective fluorescent turn-on probe HSip-1 (Cyclen-AF-Cu; Hydrogen Sulfide imaging probe-1). Once entering cells, HSip-1 DA is hydrolyzed by esterases to form HSip-1, allowing the selective detection of intracellular H2S by fluorescence (Ex 470-490 nm, Em 510-550 nm). For intracellular loading, incubate cells with 5-30 μM Hsip-1 DA in DMEM at 37 °C for 30 min before removing unincorporated probe by washing with HBSS.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable