Please see the link below to the sequence for the SHC012 - CMV-TagRFP™ Control.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp.txt
SHC012
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Plasmid DNA
Contains a gene encoding TagRFP
Synonym(s):
MISSION® Control Vectors
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| Size/SKU | Availability | Price |
|---|---|---|
1 pkg | Please contact Customer Service for Availability | 15 900,00 CZK |
About This Item
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
15 900,00 CZK
Please contact Customer Service for Availability
Quality Segment
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.
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This Item | |||
|---|---|---|---|
| description Contains a gene encoding TagRFP | description Contains a gene encoding TurboGFP, under the control of the UbC promoter | description shRNA sequence targeting eGFP | description shRNA sequence targeting luciferase |
| concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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