The Datasheet for this product can be accessed in the DOCUMENTATION section under the 'More Documents' tab. Please see the product Datasheet below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/291/243/sae1000dat-ms.pdf
SAE1000
SARS-CoV-2 Receptor Binding Domain
Spike protein RBD recombinant, expressed in HEK 293 cells
Synonym(s):
COVID-19, SARS-CoV-2, Spike Protein RBD, Spike-ACE2, coronavirus
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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Please contact Customer Service for Availability | 10 400,00 CZK |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
Mol wt:
~35 kDa
recombinant
expressed in HEK 293 cells
Quality Segment
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
~35 kDa
technique(s)
ELISA: 2 μg/mL (({Functional} RBD binds 50% of the optimal binding RBD-ACE2 ))
shipped in
ambient
storage temp.
−20°C
General description
SARS-CoV-2 belongs to Coronaviridae (CoVs) - a large family of viruses that usually cause mild to moderate upper-respiratory tract diseases.1 CoVs are single-stranded RNA viruses. They have a high mutation rate and are therefore highly diverse.1 Their hosts include human and non-human mammals, as well as birds.1
Application
The activity of this product is tested by a functional ELISA. Immobilization of Spike protein Receptor Binding Domain at 2 μg/mL (100 μL/well), allows binding of ACE2, biotin tagged (Cat. No. SAE0171). The half maximal binding (binding which produces 50% of the optimal binding response) is ≤2 μg/mL.
Biochem/physiol Actions
SARS-CoV-2 infects cells which express angiotensin converting enzyme 2 (ACE2).2 ACE2 binds to the viral envelope-associated spike (S) protein as a cellular receptor, following proteolytic cleavage of both S and ACE2 by serine proteases.3 The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2.
This product is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 25 kDa (amino acids 319- 541) and includes a C-terminal polyhistidine tag.
Physical form
This product is supplied as a lyophilized powder in phosphate buffered saline (PBS), pH 7.4, with no carrier proteins.
Preparation Note
Store the product at –20 °C. The product retains its activity for at least 2 years as supplied. After initial thawing, it is recommended to store the protein in working aliquots at –20 °C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Data presented is the available current product information and provided as-is. This product has not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.
Data presented is the available current product information and provided as-is. This product has not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.
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This Item | |||
|---|---|---|---|
| description Spike protein RBD recombinant, expressed in HEK 293 cells | description - | description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution |
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| recombinant expressed in HEK 293 cells | recombinant - | recombinant - | recombinant - |
| technique(s) ELISA: 2 μg/mL (({Functional} RBD binds 50% of the optimal binding RBD-ACE2 )) | technique(s) ELISA: suitable, western blot: suitable, neutralization: suitable | technique(s) immunoblotting: 1:2000-1:4000 using SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating., ELISA: 1/25-1/200 using 2 μg/mL SARS-CoV-2 RBD (Cat. No. SAE1000) for coating and 2 μg/ml ACE2-Biotin (Cat. No. SAE0171) for competition ((Neutralizing ELISA)), indirect ELISA: 1/2000-1/4000 using 2 μg/mL SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating | technique(s) immunoblotting: 1:1000-1:2000 using SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating., indirect ELISA: 1/2000-1/4000 using 2 μg/mL SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating. |
| mol wt ~35 kDa | mol wt calculated mol wt 139.13 kDa, observed mol wt ~38 kDa | mol wt - | mol wt - |
| form lyophilized powder | form - | form liquid | form liquid |
| storage temp. −20°C | storage temp. - | storage temp. −20°C | storage temp. −20°C |
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Hello, I cannot find the user guidelines for this product. It will be helpful if anyone can describe the application of this product in detail. It's a little bit confusing for me. Thank you in advance.
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