SAE0175

MMP-2 pre-activated human

recombinant, ≥1,000 pmol/min/μg, expressed in HEK 293 cells

• Synonym(s): 72 kDa gelatinase, Gelatinase A, MMP-2, Matrix metalloproteinase-2, TBE-1
• NACRES: NA.32
• UNSPSC Code: 12352202
• Specific activity: ≥1000 pmol/min-μg
• Recombinant: expressed in HEK 293 cells

Properties

• recombinant: expressed in HEK 293 cells
• Quality Segment: 200
• specific activity: ≥1000 pmol/min-μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Matrix Metalloproteinase-2 (MMP-2) is a member of the matrix metalloproteinase (MMP) family of proteins. MMPs participate in the breakdown of extracellular matrix in normal physiological processes like embryonic development, reproduction, and tissue remodeling, as well as in disease processes such as arthritis and metastasis. MMP-2 cleaves many substrates, including extracellular matrix components (collagens, fibronectin, and elastin), soluble metabolic mediators (e.g., apolipoproteins), secreted and extracellular matrix-anchored growth factors, and cytokines.

Along with MMP-9, MMP-2 is involved many pathophysiological processes, including leukocyte migration from the circulation into the tissue during inflammation, Chagas′ Cardiomyopathy, heart failure and chronic kidney disease. MMP-2 thus may be regarded as a potential therapeutic target.

As with most MMPs, MMP-2 is secreted as an inactive pro-protein, which becomes activated when cleaved by extracellular proteinases. This product was pre-activated in vitro using 4-aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. The highly toxic APMA was removed from the final preparation.

This product is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 72 kDa (amino acids 110-660). The DTT-reduced protein migrates as a 75-80 kDa polypeptide on SDS-PAGE because of glycosylation. This protein is produced in human cells, without the use of serum. The human cells expression system allows human like glycosylation and folding, and often supports higher specific activity of the protein. This recombinant protein is expressed without artificial tags.

Features and Benefits

• Highly purified protein without artificial fusion tags
• Expressed in human cells (HEK 293) for proper glycosylation
• Pre-activated and ready to use
• High substrate activity

Physical form

This product is supplied as a 0.22 μm-filtered solution, containing 20 mM Trizma^®, pH 7.5, containing 8 mM CaCl2, 119 mM NaCl, 20% glycerol, and 0.05% Brij^® 35.

Preparation Note

Store the product at –70 °C. The product retains its activity for at least 2 years as supplied. After initial thawing, it is recommended to store the protein in working aliquots at –70 °C.

Other Notes

This product was pre-activated in vitro using 4-Aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. In order to save our customers from handling hazardous materials, and for environmental saving, the highly toxic and fatal APMA was removed from the final preparation.

Legal Information

Brij is a registered trademark of Croda International PLC

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is for R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1