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SAE0159

Microbial Transglutaminase

lyophilized powder, ≥12 units/mg protein

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Synonym(s):

Microbial Enzyme, Transglutaminase, Transglutaminase Enzyme

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Size/SKUAvailabilityPrice
25 units
Please contact Customer Service for Availability
9 040,00 CZK
250 units
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70 300,00 CZK
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382 500,00 CZK

About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥12 units/mg protein

9 040,00 CZK

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form

lyophilized powder

Quality Segment

specific activity

≥12 units/mg protein

shipped in

dry ice

storage temp.

−20°C

General description

Transglutaminases (TG) are a family of enzymes that catalyze isopeptide bond formation. This bond formation occurs between the γ-carboxyamide group of glutamine and various primary amines (primarily the ε-amino group of lysine). The resulting intermolecular or intramolecular cross-linking of transglutaminase is highly stable and shows high resistance to proteolytic degradation. The transglutaminase crosslinking activity support the formation of blood clots , skin and hair. On the other hand, TG is now being implicated in Celiac disease as well as in Huntington and Parkinson diseases. Historically, Microbial transglutaminase has been heavily used in the food industry. Microbial TG is often preferred for newer applications (such as site-specific protein modification and antibody drug conjugation) due to its small molecular weight and lack of calcium dependency when compared to the mammalian forms.

Application

Microbial Transglutaminase has been used for:
  • Protein cross-linking & site-specific labeling
  • Antibody Drug Conjugation
  • 3D bioprinting bioink preparation
  • Food related immunogenicity/pathogenicity related research

Features and Benefits

  • Small (~38kDa) and calcium independent enzyme
  • Highly purified lyophilized enzyme
  • Consistent and reproducible activity
  • Characterized for endotoxin content

Preparation Note

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices
Store the freeze-dried product at –20 °C. It is recommended to store the reconstituted protein in working aliquots at –20 °C to avoid repeated freeze/ thaw cycles.

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This Item
616467WH0007052M10SAB4502963
Microbial Transglutaminase lyophilized powder, ≥12 units/mg protein

Sigma-Aldrich

SAE0159

Microbial Transglutaminase

Transglutaminase 2 Inhibitor, ZDON A cell-permeable, peptide-based, irreversible inhibitor of transglutaminase 2 (TG2; IC50 = 150 nM for recombinant TG2). Reacts with the active site cysteine of TG2.

Sigma-Aldrich

616467

Transglutaminase 2 Inhibitor, ZDON

Monoclonal Anti-TGM2 antibody produced in mouse clone 2F4, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0007052M10

Monoclonal Anti-TGM2 antibody produced in mouse

Anti-Transglutaminase 2, N-Terminal antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4502963

Anti-Transglutaminase 2, N-Terminal antibody produced in rabbit

description

lyophilized powder, ≥12 units/mg protein

description

A cell-permeable, peptide-based, irreversible inhibitor of transglutaminase 2 (TG2; IC50 = 150 nM for recombinant TG2). Reacts with the active site cysteine of TG2.

description

clone 2F4, purified immunoglobulin, buffered aqueous solution

description

affinity isolated antibody

specific activity

≥12 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

lyophilized powder

form

powder

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000337508
    0000427692
    0000452349
    0000549707
    0000550305

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    Questions

    1–3 of 3 Questions  

    1. Hi, I have purchased Microbial Transglutaminase lyophilized powder, >12 units per mg (SAE0159-25UN). How can I prepare its stock? How much volume of solvent (for example PBS) should I add in the vial containing enzyme powder? 

      1 answer

      1. Reconstitute this product in 20 mM Tris-HCl containing 150 mM NaCl, in Molecular Biology grade water, pH 8 at 0.14 units per microliter. The unit per milligram activity is lot-specific and available in the product Certificate of Analysis. Further usage instructions are available in the product datasheet at the link below:
        https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/339/389/sae0159pis-ms.pdf

        Please see the following link to review a sample or lot-specific Certificate:
        https://www.sigmaaldrich.com/US/en/product/sigma/sae0159*product-documentation

        Helpful?

    2. Bonjour, Est-il possible de savoir la masse totale d'enzyme dans le produit ? Les 25 units correspondent bien à l'activité de la poudre (mélange enzyme + excipients) ?

      1 answer

      1. The total mass of product in each vial varies from lot to lot due to variability in the protein content and the activity per milligram protein. The vial contents may be calculated based on information reported in the Certificate of Analysis. A review of recent lots reveals a range of 14 - 30% protein with an activity range of 14 -38 units per milligram protein.

        Please see the link below to review a sample or lot specific Certificate:
        https://www.sigmaaldrich.com/product/sigma/sae0159#product-documentation

        Please see the following link to review the product data sheet:
        https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/339/389/sae0159pis-ms.pdf

        Helpful?

    3. こちらの製品は大腸菌由来のリコンビナントタンパクでしょうか？

      1 answer

      1. This is a cell culture fermentation product. The source organism is considered proprietary.

        Helpful?

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